Many times you have seen us post articles urging private well owners to test for the presence of bacteria in their wells on a regular basis — ‘cuz no one else will ever volunteer to do it for them. We have even given links to a basic bacteria in water test kit from two different companies (SenSafe & WaterSafe) and to a more advanced bacteria test kit from SenSafe that lets a person find out not only if they have coliform bacteria present in their water, but also if they have E. Coli present.
Today we have information about the ‘tools’ required to take full advantage of the advanced bacteria test kit from SenSafe and how to properly use them to test for the presence of coliform bacteria in water samples.
How to use the Sensafe EZ Coliform Cult E.Coli Test Kit
- First of all, make sure to keep the test kit dry and in temperatures of 15-27 C (59-81 F).
- Wash hands thoroughly with soap and water before gathering your water sample. Dry hands completely.
- Carefully remove the bottle cap from the Sensafe EZ Coliform Cult E.Coli Test Kit.
- Add 100 milliliters of sample water but make sure not to overfill.
- Quickly put the cap back on the test bottle and secure it tightly.
- Shake test bottle vigorously until all of the media (solids inside the bottle) dissolve. Solution should turn a clear yellow color at this point. Turbid samples will have a less clear appearance.
- Either incubate the sample for 24 hours at 35 deg C/95 deg F — or — for 48 hours at 25 deg C/77 deg F.
- At the end of incubation period, look at the color of the sample. A yellow color indicates a lack of coliforms while a bluish green color indicates the presence of coliforms.
For those who wish to learn if their water sample contains E. Coli, two options exist:
- Shine a UV light with wavelength 365nm from the bottom of the sample. If bright blue fluorescence occurs, the sample contains E. Coli. Where can you obtain a UV light source to perform this test? WaterFilters.Net carries the Sensafe NVF-4 Handheld UV Light.
- Addition of Sensafe Kovacs Indole Reagent will also let you know if the sample contains E. Coli. Unfortunately we do not, at this time, have specific information regarding how this process works.